ARHGAP30 Antibody

ProSci
Product Code: PSI-61-747
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-747-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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(LEFT)Western blot analysis of ARHGAP30 Antibody in Ramos cell line lysates (35ug/lane).ARHGAP30 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of ARHGAP30 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane).ARHGAP30 (arrow) was detected using the purified Pab.
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Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

(LEFT)Western blot analysis of ARHGAP30 Antibody in Ramos cell line lysates (35ug/lane).ARHGAP30 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of ARHGAP30 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane).ARHGAP30 (arrow) was detected using the purified Pab.
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Rho GTPase-activating protein 30, Rho-type GTPase-activating protein 30, ARHGAP30
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ARHGAP30 is GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Background References:
  • Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
  • Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ARHGAP30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-812 amino acids from the C-terminal region of human ARHGAP30.
NCBI Gene ID #:
257106
NCBI Official Name:
Rho GTPase-activating protein 30
NCBI Official Symbol:
ARHGAP30
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
119 kDa
Protein Accession #:
Q7Z6I6
Protein GI Number:
334302880
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q7Z6I6
User NOte:
Optimal dilutions for each application to be determined by the researcher.