ACER3 Antibody

ProSci
Product Code: PSI-63-718
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-718-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ACER3 Antibody in HepG2 cell line lysates (35ug/lane)
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Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ACER3 Antibody in HepG2 cell line lysates (35ug/lane)
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Alkaline ceramidase 3, AlkCDase 3, Alkaline CDase 3, 351-, Alkaline dihydroceramidase SB89, Alkaline phytoceramidase, aPHC, ACER3, APHC, PHCA
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ACER3 hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.
Background References:
  • Wheeler,H.E., et.al., PLoS Genet.(10), E1000685 (2009)
  • Mao,C. et.al., Biochim. Biophys. Acta 1781 (9), 424-434 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ACER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 amino acids from the C-terminal region of human ACER3.
NCBI Gene ID #:
55331
NCBI Official Name:
Alkaline ceramidase 3
NCBI Official Symbol:
ACER3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
Q9NUN7
Protein GI Number:
296439452
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9NUN7
User NOte:
Optimal dilutions for each application to be determined by the researcher.