PDE1B Antibody

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ProSci
Product Code: PSI-56-074
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-074-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in mouse brain tissue lysates (35ug/lane).
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Western Blot at 1:1000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse brain tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, Cam-PDE 1B, 63 kDa Cam-PDE, PDE1B, PDE1B1, PDES1B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Vandeput, F., et al. J. Biol. Chem. 282(45):32749-32757(2007)
  • Bender, A.T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(2):460-465(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-500 amino acids from the C-terminal region of human PDE1B.
NCBI Gene ID #:
5153
NCBI Official Name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
NCBI Official Symbol:
PDE1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q01064
Protein GI Number:
3183514
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q01064
User NOte:
Optimal dilutions for each application to be determined by the researcher.