DOK5 Antibody

ProSci
Product Code: PSI-63-151
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-151-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Antibody is used in Western blot to detect DOK5 in mouse liver tissue lysate.
2 / 3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
3 / 3

Antibody is used in Western blot to detect DOK5 in mouse liver tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Docking protein 5, Downstream of tyrosine kinase 5, Insulin receptor substrate 6, IRS-6, IRS6, DOK5, C20orf180
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
DOK5 is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP.
Background References:
  • Cai, D., et al., J. Biol. Chem. 278(28):25323-25330 (2003).
  • Favre, C., et al., Genes Immun. 4(1):40-45 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DOK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-306 amino acids from the C-terminal region of human DOK5.
NCBI Gene ID #:
55816
NCBI Official Name:
Docking protein 5
NCBI Official Symbol:
DOK5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
Q9P104
Protein GI Number:
26393190
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q9P104
User NOte:
Optimal dilutions for each application to be determined by the researcher.