Diacylglycerol kinase iota Antibody

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ProSci
Product Code: PSI-63-406
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-406-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect DGKI in mouse cerebellum tissue lysate.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Antibody is used in Western blot to detect DGKI in mouse cerebellum tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Diacylglycerol kinase iota, DAG kinase iota, Diglyceride kinase iota, DGK-iota, DGKI
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
DGKI a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.
Background References:
  • Ding, L., et al., J. Biol. Chem. 273(49):32746-32752 (1998).
  • Bowne, S.J., et al., Mol. Vis. 6, 6-9 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Diacylglycerol kinase iota (DGKI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 890-920 amino acids from the C-terminal region of human Diacylglycerol kinase iota (DGKI).
NCBI Gene ID #:
9162
NCBI Official Name:
Diacylglycerol kinase iota
NCBI Official Symbol:
DGKI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
117 kDa
Protein Accession #:
O75912
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O75912
User NOte:
Optimal dilutions for each application to be determined by the researcher.