KRT82 Antibody

ProSci
Product Code: PSI-61-771
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-771-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of KRT82 Antibody in mouse heart tissue lysates (35ug/lane)
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KRT82 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of KRT82 Antibody in mouse heart tissue lysates (35ug/lane)
KRT82 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Keratin, type II cuticular Hb2, Keratin-82, K82, Type II hair keratin Hb2, Type-II keratin Kb22, KRT82, KRTHB2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
KRT82 is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this keratin appears to be a hair cuticle-specific keratin.
Background References:
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006) Rogers, M.A., et al. J. Invest. Dermatol. 124(3):536-544(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KRT82 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-341 amino acids from the Central region of human KRT82.
NCBI Gene ID #:
3888
NCBI Official Name:
Keratin, type II cuticular Hb2
NCBI Official Symbol:
KRT82
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q9NSB4
Protein GI Number:
148887391
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9NSB4
User NOte:
Optimal dilutions for each application to be determined by the researcher.