ANXA2 Antibody

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ProSci
Product Code: PSI-62-900
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-900-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining ANXA2 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lane 4: SH-SY5Y whole cell lysates Lane 5: SK-BR-3 whole cell lysates Lysates/proteins at 20 ug per lane.
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Western blot analysis of anti-ANXA2 Antibody in mouse lung tissue lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).
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Antibody staining ANXA2 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lane 4: SH-SY5Y whole cell lysates Lane 5: SK-BR-3 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-ANXA2 Antibody in mouse lung tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, PAP-IV, Protein I, p36, ANXA2, ANX2, ANX2L4, CAL1H, LPC2D
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
ANXA2 is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.
Background References:
  • He,K.L., J. Biol. Chem. 283 (28), 19192-19200 (2008) Tamma,G., Pflugers Arch. 456 (4), 729-736 (2008) Gou,D., J. Biol. Chem. 283 (19), 13156-13164 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig, Rat
Immunogen:
This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human ANXA2.
NCBI Gene ID #:
302
NCBI Official Name:
Annexin A2
NCBI Official Symbol:
ANXA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
P07355
Protein GI Number:
113950
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P07355
User NOte:
Optimal dilutions for each application to be determined by the researcher.