SSR1 Antibody

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ProSci
Product Code: PSI-57-743
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-743-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SSR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of SSR1 using rabbit SSR1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SSR1 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SSR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of SSR1 using rabbit SSR1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SSR1 gene (Lane 2).

Documents

Further Information

Additional Names:
Translocon-associated protein subunit alpha, TRAP-alpha, Signal sequence receptor subunit alpha, SSR-alpha, SSR1, TRAPA
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are exclusively non-canonical.
Background References:
  • Hirama, T., et al., FEBS Lett. 455(3):223-227 (1999).
  • Hartmann, E., et al., FEBS Lett. 349(3):324-326 (1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human SSR1.
NCBI Gene ID #:
6745
NCBI Official Name:
Translocon-associated protein subunit alpha
NCBI Official Symbol:
SSR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
P43307
Protein GI Number:
22261821
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P43307
User NOte:
Optimal dilutions for each application to be determined by the researcher.