AWAT2 Antibody

ProSci
Product Code: PSI-55-450
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-450-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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AWAT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse liver,spleen tissue lysates (35ug/lane).

AWAT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse liver,spleen tissue lysates (35ug/lane).

Further Information

Additional Names:
Acyl-CoA wax alcohol acyltransferase 2, Acyl-CoA retinol O-fatty-acyltransferase, ARAT, Retinol O-fatty-acyltransferase, Diacylglycerol O-acyltransferase 2-like protein 4, Diacylglycerol O-acyltransferase candidate 4, hDC4, Long-chain-alcohol O-fatty-acyltransferase 2, Multifunctional O-acyltransferase, Wax synthase, hWS, AWAT2, DC4, DGAT2L4, MFAT, WS
Application Note:
For IHC-P starting dilution is: 1:50~100

For WB starting dilution is: 1:1000
Background:
This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters.
Background References:
  • Holmes, R.S. Comp. Biochem. Physiol. PartGenomics Proteomics 5(1):45-54(2010)
  • Yen, C.L., et al. J. Lipid Res. 46(11):2388-2397(2005)
  • Turkish, A.R., et al. J. Biol. Chem. 280(15):14755-14764(2005)
  • Cheng, J.B., et al. J. Biol. Chem. 279(36):37798-37807(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AWAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-331 amino acids from the C-terminal region of human AWAT2.
NCBI Gene ID #:
158835
NCBI Official Name:
Acyl-CoA wax alcohol acyltransferase 2
NCBI Official Symbol:
AWAT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q6E213
Protein GI Number:
74748433
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q6E213
User NOte:
Optimal dilutions for each application to be determined by the researcher.