SEC61B Antibody

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ProSci
Product Code: PSI-58-090
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-090-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse liver tissue lysates (35ug/lane).
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SEC61B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse liver tissue lysates (35ug/lane).
SEC61B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Protein transport protein Sec61 subunit beta, SEC61B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
Background References:
  • Liao, H.J., et al. Cancer Res. 69(15):6179-6183(2009)
  • Wang, B., et al. Cell 133(6):1080-1092(2008)
  • Liao, H.J., et al. Mol. Biol. Cell 18(3):1064-1072(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SEC61B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the Central region of human SEC61B.
NCBI Gene ID #:
10952
NCBI Official Name:
Protein transport protein Sec61 subunit beta
NCBI Official Symbol:
SEC61B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
10 kDa
Protein Accession #:
P60468
Protein GI Number:
42560366
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P60468
User NOte:
Optimal dilutions for each application to be determined by the researcher.