SCYL2 Antibody

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ProSci
Product Code: PSI-62-598
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-598-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-SCYL2 Pab (AP7024b) in T47D (left) and mouse brain (right) tissue lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with SCYL2 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-SCYL2 Pab (AP7024b) in T47D (left) and mouse brain (right) tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with SCYL2 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
SCY1-like protein 2, Coated vesicle-associated kinase of 104 kDa, SCYL2, CVAK104, KIAA1360
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
SCYL2 is a component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. SCYL2 is a probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. SCYL2 may also regulate clathrin-dependent trafficking between the TGN and/or the endosomal system.
Background References:
  • Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SCYL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-672 amino acids from the C-terminal region of human SCYL2.
NCBI Gene ID #:
55681
NCBI Official Name:
SCY1-like protein 2
NCBI Official Symbol:
SCYL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
104 kDa
Protein Accession #:
Q6P3W7
Protein GI Number:
74762350
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q6P3W7
User NOte:
Optimal dilutions for each application to be determined by the researcher.