ARHGDIA Antibody

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ProSci
Product Code: PSI-61-585
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-585-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H.liver section using ARHGDIA Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Western blot analysis in A2058,Hela cell line and mouse lung lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H.liver section using ARHGDIA Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis in A2058,Hela cell line and mouse lung lysates (35ug/lane).

Documents

Further Information

Additional Names:
Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-GDI alpha, ARHGDIA, GDIA1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
ARHGDIA belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA
Background References:
  • Qiao,J., etc, Am. J. Physiol., Cell Physiol. 295 (5), C1161-C1168 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Rat
Immunogen:
This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-53 amino acids from the N-terminal region of human ARHGDIA.
NCBI Gene ID #:
396
NCBI Official Name:
Rho GDP-dissociation inhibitor 1
NCBI Official Symbol:
ARHGDIA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
P52565
Protein GI Number:
1707892
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P52565
User NOte:
Optimal dilutions for each application to be determined by the researcher.