CHST3 Antibody

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ProSci
Product Code: PSI-55-191
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-191-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse spleen and lung tissue lysates (35ug/lane).
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CHST3 antibodyimmunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHST3 antibodyfor immunohistochemistry.
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Western blot analysis in mouse spleen and lung tissue lysates (35ug/lane).
CHST3 antibodyimmunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHST3 antibodyfor immunohistochemistry.

Documents

Further Information

Additional Names:
Carbohydrate sulfotransferase 3, Chondroitin 6-O-sulfotransferase 1, C6ST-1, Chondroitin 6-sulfotransferase, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0, GST-0, CHST3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CHST3 is an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation.
Background References:
  • Unger, S., et al. Am. J. Med. Genet.152A (10), 2543-2549 (2010) :
  • Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
  • Tuysuz, B., et al. Clin. Genet. 75(4):375-383(2009)
  • Kitagawa, H., et al. J. Biol. Chem. 283(41):27438-27443(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This CHST3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human CHST3.
NCBI Gene ID #:
9469
NCBI Official Name:
Carbohydrate sulfotransferase 3
NCBI Official Symbol:
CHST3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
Q7LGC8
Protein GI Number:
116241297
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q7LGC8
User NOte:
Optimal dilutions for each application to be determined by the researcher.