STK35 Antibody

ProSci
Product Code: PSI-62-756
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-756-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Antibody is used in Western blot to detect STK35 in mouse kidney tissue lysate.
2 / 3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
3 / 3

Antibody is used in Western blot to detect STK35 in mouse kidney tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Serine/threonine-protein kinase 35, CLP-36-interacting kinase 1, CLIK-1, PDLIM1-interacting kinase 1, Serine/threonine-protein kinase 35 L1, STK35, CLIK1, PDIK1, STK35L1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
STK35 can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded kinase may be a regulator of actin stress fibers in nonmuscle cells. This protein has also been found in the nucleus.
Background References:
  • Vallenius, T., et al., J. Cell. Sci. 115 (Pt 10), 2067-2073 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This STK35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-404 amino acids from the Central region of human STK35.
NCBI Gene ID #:
140901
NCBI Official Name:
Serine/threonine-protein kinase 35
NCBI Official Symbol:
STK35
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
Q8TDR2
Protein GI Number:
292495039
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q8TDR2
User NOte:
Optimal dilutions for each application to be determined by the researcher.