COL5A1 Antibody

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ProSci
Product Code: PSI-55-212
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-212-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in U251 cell line lysates (35ug/lane).
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Western blot analysis in U251 cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Collagen alpha-1(V) chain, COL5A1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq].
Background References:
  • Vitart, V., et al. Hum. Mol. Genet. 19(21):4304-4311(2010)
  • Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Posthumus, M., et al. Med Sci Sports Exerc (2010) In press :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human COL5A1.
NCBI Gene ID #:
1289
NCBI Official Name:
Collagen alpha-1(V) chain
NCBI Official Symbol:
COL5A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
184 kDa
Protein Accession #:
P20908
Protein GI Number:
85687376
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P20908
User NOte:
Optimal dilutions for each application to be determined by the researcher.