GLG1 Antibody

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ProSci
Product Code: PSI-64-142
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-64-142-400ul400ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: MCF-7 lysate Lane 2: HepG2 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: human placenta lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in HepG2,T47D cell line lysates (35ug/lane).
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Western blot analysis of GLG1 Antibody in mouse cerebellum tissue lysates (35ug/lane)
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GLG1 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: MCF-7 lysate Lane 2: HepG2 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: human placenta lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HepG2,T47D cell line lysates (35ug/lane).
Western blot analysis of GLG1 Antibody in mouse cerebellum tissue lysates (35ug/lane)
GLG1 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Golgi apparatus protein 1, CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160, GLG1, CFR1, ESL1, MG160
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100
Background References:
  • Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
  • Kibriya, M.G., et al. Breast Cancer Res. Treat. 114(3):463-477(2009)
  • Antoine, M., et al. Oncol. Rep. 21(2):357-362(2009)
  • Ahn, J., et al. J. Cell. Sci. 118 (PT 8), 1725-1731 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Hamster, Chicken
Immunogen:
This GLG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1179 amino acids from the C-terminal region of human GLG1.
NCBI Gene ID #:
2734
NCBI Official Name:
Golgi apparatus protein 1
NCBI Official Symbol:
GLG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
135 kDa
Protein Accession #:
Q92896
Protein GI Number:
218512060
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q92896
User NOte:
Optimal dilutions for each application to be determined by the researcher.