SIL1 Antibody

ProSci
Product Code: PSI-63-496
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-496-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of SIL1 Antibody in mouse kidney tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SIL1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of SIL1 Antibody in mouse kidney tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SIL1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Nucleotide exchange factor SIL1, BiP-associated protein, BAP, SIL1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
SIL1 IS a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein.
Background References:
  • Clark,H.F., ET.AL., Genome Res. 13 (10), 2265-2270 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-126 amino acids from the Central region of human SIL1.
NCBI Gene ID #:
64374
NCBI Official Name:
Nucleotide exchange factor SIL1
NCBI Official Symbol:
SIL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
Q9H173
Protein GI Number:
74733533
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9H173
User NOte:
Optimal dilutions for each application to be determined by the researcher.