BLOC1S2 Antibody

ProSci
Product Code: PSI-61-967
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-967-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HepG2 cell line lysates (15ug/lane).
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Western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).
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Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in HepG2 cell line lysates (15ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Biogenesis of lysosome-related organelles complex 1 subunit 2, BLOC-1 subunit 2, Centrosome-associated protein, BLOC1S2, BLOS2, CEAP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
BLOC1S2 is a component of the ubiquitously expressed BLOC1 multisubunit protein complex. BLOC1 is required for normal biogenesis of specialized organelles of the endosomal-lysosomal system, such as melanosomes and platelet dense granules (Starcevic and Dell'Angelica, 2004 [PubMed 15102850]).
Background References:
  • Rodriguez-Fernandez, I.A., et al. J. Inherit. Metab. Dis. 32(2):190-203(2009)
  • Gdynia, G., et al. Apoptosis 13(3):437-447(2008)
  • Wang, Z., et al. J. Mol. Biol. 343(1):71-82(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This BLOC1S2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 amino acids from the Central region of human BLOC1S2.
NCBI Gene ID #:
282991
NCBI Official Name:
Biogenesis of lysosome-related organelles complex 1 subunit 2
NCBI Official Symbol:
BLOC1S2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
Q6QNY1
Protein GI Number:
74749300
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q6QNY1
User NOte:
Optimal dilutions for each application to be determined by the researcher.