EHD3 Antibody

ProSci
Product Code: PSI-62-928
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-928-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis of EHD3 Antibody in mouse lung tissue lysates (35ug/lane)
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4 / 4
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of EHD3 Antibody in mouse lung tissue lysates (35ug/lane)
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
EH domain-containing protein 3, PAST homolog 3, EHD3, EHD2, PAST3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
EHD3 plays a role in endocytic transport.
Background References:
  • Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
  • Naslavsky, N., et al. J. Cell. Sci. 122 (PT 3), 389-400 (2009)
  • Roland, J.T., et al. Mol. Biol. Cell 18(8):2828-2837(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of human EHD3.
NCBI Gene ID #:
30845
NCBI Official Name:
EH domain-containing protein 3
NCBI Official Symbol:
EHD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q9NZN3
Protein GI Number:
300669636
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q9NZN3
User NOte:
Optimal dilutions for each application to be determined by the researcher.