HSPD1 Antibody

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ProSci
Product Code: PSI-61-555
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-555-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A431,mouse NIH/3T3 cell line and mouse liver tissue lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in A431,mouse NIH/3T3 cell line and mouse liver tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.
Background References:
  • Venner T.J., Singh B., Gupta R.S.DNA Cell Biol. 9:545-552(1990)
  • Hansen J.J., Bross P., Westergaard M., Nielsen M.N., Eiberg H.,Hum. Genet. 112:71-77(2003)
  • Rasmussen R.K., Ji H., Eddes J.S., Moritz R.L.,Electrophoresis 18:588-598(1997)
  • Aboulaich N., Vainonen J.P., Stralfors P., Vener A.V.Biochem. J. 383:237-248(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Hamster, Rat
Immunogen:
This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the C-terminal region of human HSPD1.
NCBI Gene ID #:
3329
NCBI Official Name:
60 kDa heat shock protein, mitochondrial
NCBI Official Symbol:
HSPD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
P10809
Protein GI Number:
129379
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P10809
User NOte:
Optimal dilutions for each application to be determined by the researcher.