Mouse Map4k3 Antibody

ProSci
Product Code: PSI-57-738
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-738-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HepG2 cell line lysates (35ug/lane).
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
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Mouse Map4k3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Mouse Map4k3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Mitogen-activated protein kinase kinase kinase kinase 3, Germinal center kinase-related protein kinase, GLK, MAPK/ERK kinase kinase kinase 3, MEK kinase kinase 3, MEKKK 3, Map4k3
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
Map4k3 may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Map4k3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 501-529 amino acids from the Central region of mouse Map4k3.
NCBI Gene ID #:
225028
NCBI Official Name:
Mitogen-activated protein kinase kinase kinase kinase 3
NCBI Official Symbol:
Map4k3
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
101 kDa
Protein Accession #:
Q99JP0
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q99JP0
User NOte:
Optimal dilutions for each application to be determined by the researcher.