HtrA1 Antibody

ProSci
Product Code: PSI-56-986
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-986-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.
2 / 3
HtrA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.
HtrA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Serine protease HTRA1, 3421-, High-temperature requirement A serine peptidase 1, L56, Serine protease 11, HTRA1, HTRA, PRSS11
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
HtrA1 a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth.
Background References:
  • Howes, N., et al., Clin Gastroenterol Hepatol 2(3):252-261 (2004).
  • Chien, J., et al., Oncogene 23(8):1636-1644 (2004).
  • Hu, S.I., et al., J. Biol. Chem. 273(51):34406-34412 (1998).
  • Zumbrunn, J., et al., Genomics 45(2):461-462 (1997).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HtrA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-147 amino acids from the N-terminal region of human HtrA1.
NCBI Gene ID #:
5654
NCBI Official Name:
Serine protease HTRA1
NCBI Official Symbol:
HTRA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q92743
Protein GI Number:
18202620
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q92743
User NOte:
Optimal dilutions for each application to be determined by the researcher.