TEC Antibody

Contact us
ProSci
Product Code: PSI-63-168
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-168-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Fluorescent image of Hela cell stained with TEC Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
2 / 5
Fluorescent image of Hela cell stained with TEC Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
3 / 5
4 / 5
Western blot analysis of anti-TEC Pab in mouse liver tissue lysate
5 / 5
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
❮❯

Fluorescent image of Hela cell stained with TEC Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
Fluorescent image of Hela cell stained with TEC Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of anti-TEC Pab in mouse liver tissue lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Tyrosine-protein kinase Tec, TEC, PSCTK4
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
TEC belongs to the TEC subfamily of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. TEC family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. TEC kinase is an integral component of T cell signaling and has a distinct role in T cell activation. TEC may be associated with myelodysplastic syndrome.
Background References:
  • Nore, B.F., et al., Biochim. Biophys. Acta 1645(2):123-132 (2003).
  • Maltais, A., et al., Cancer Lett. 183(1):87-94 (2002).
  • Lachance, G., et al., J. Biol. Chem. 277(24):21537-21541 (2002).
  • Yang, W.C., et al., Immunity 12(4):373-382 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TEC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-205 amino acids from the Central region of human TEC.
NCBI Gene ID #:
7006
NCBI Official Name:
Tyrosine-protein kinase Tec
NCBI Official Symbol:
TEC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
74 kDa
Protein Accession #:
P42680
Protein GI Number:
158518392
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P42680
User NOte:
Optimal dilutions for each application to be determined by the researcher.