KLF6 Antibody

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ProSci
Product Code: PSI-62-347
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-347-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of KLF6 Antibody in mouse stomach tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Fluorescent confocal image of Hela cell stained with KLF6 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.
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Western blot analysis of KLF6 Antibody in mouse stomach tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Fluorescent confocal image of Hela cell stained with KLF6 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Krueppel-like factor 6, B-cell-derived protein 1, Core promoter element-binding protein, GC-rich sites-binding factor GBF, Proto-oncogene BCD1, Suppressor of tumorigenicity 12 protein, Transcription factor Zf9, KLF6, BCD1, COPEB, CPBP, ST12
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
COPEB is a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor.
Background References:
  • Sangodkar,J., Eur. J. Cancer 45 (4), 666-676 (2009) DiFeo,A., Drug Resist. Updat. 12 (1-2), 1-7 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KLF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of human KLF6.
NCBI Gene ID #:
1316
NCBI Official Name:
Krueppel-like factor 6
NCBI Official Symbol:
KLF6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
Q99612
Protein GI Number:
7387592
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q99612
User NOte:
Optimal dilutions for each application to be determined by the researcher.