MAPK11 Antibody

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ProSci
Product Code: PSI-62-738
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-738-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-MAPK11 Pab in mouse brain tissue lysate
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of anti-MAPK11 Pab in mouse brain tissue lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Mitogen-activated protein kinase 11, MAP kinase 11, MAPK 11, Mitogen-activated protein kinase p38 beta, MAP kinase p38 beta, p38b, Stress-activated protein kinase 2b, SAPK2b, p38-2, MAPK11, PRKM11, SAPK2, SAPK2B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
MAPK11 is a Ser/Thr kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. It preferentially phosphorylates transcription factor ATF2. This protein is activated by phosphorylation on threonine and tyrosine by MKK6, and is inhibited by pyridinyl-imidazole related compounds. Highest expression levels are in the brain and heart, with additional expression in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
Background References:
  • Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
  • Dunham, I., et al., Nature 402(6761):489-495 (1999).
  • Enslen, H., et al., J. Biol. Chem. 273(3):1741-1748 (1998).
  • Kumar, S., et al., Biochem. Biophys. Res. Commun. 235(3):533-538 (1997).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MAPK11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334-366 amino acids from the center region of human MAPK11.
NCBI Gene ID #:
5600
NCBI Official Name:
Mitogen-activated protein kinase 11
NCBI Official Symbol:
MAPK11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
Q15759
Protein GI Number:
134047835
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction,
Swissprot #:
Q15759
User NOte:
Optimal dilutions for each application to be determined by the researcher.