SMYD2 Antibody

ProSci
Product Code: PSI-55-183
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-183-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: HT-1080 whole cell lysates Lane 2: mouse heart lysates Lane 3: Hela whole cell lysates Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis of SYMD2 using rabbit polyclonal SYMD2 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SYMD2 gene (Lane 2).
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

Western Blot at 1:2000 dilution Lane 1: HT-1080 whole cell lysates Lane 2: mouse heart lysates Lane 3: Hela whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of SYMD2 using rabbit polyclonal SYMD2 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SYMD2 gene (Lane 2).
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

Further Information

Additional Names:
N-lysine methyltransferase SMYD2, 211-, HSKM-B, Histone methyltransferase SMYD2, Lysine N-methyltransferase 3C, SET and MYND domain-containing protein 2, SMYD2, KMT3C
Application Note:
For WB starting dilution is: 1:1000
Background:
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM].
Background References:
  • Abu-Farha,M.,Mol. Cell Proteomics(3), 560-572 (2008)
  • Ewing,R.M.,Mol. Syst. Biol. 3, 89 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This SMYD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-152 amino acids from the N-terminal region of human SMYD2.
NCBI Gene ID #:
56950
NCBI Official Name:
N-lysine methyltransferase SMYD2
NCBI Official Symbol:
SMYD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q9NRG4
Protein GI Number:
90185234
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9NRG4
User NOte:
Optimal dilutions for each application to be determined by the researcher.