MEKK6 Antibody

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ProSci
Product Code: PSI-63-283
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-283-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect MEKK6 in NIH3T3 cell lysate.
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Antibody is used in Western blot to detect MEKK6 in NIH3T3 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Mitogen-activated protein kinase kinase kinase 6, Apoptosis signal-regulating kinase 2, MAP3K6, ASK2, MAPKKK6, MEKK6
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK6 activates the JNK, but not ERK or p38 kinase pathways.
Background References:
  • Wang, X.S., et al., Biochem. Biophys. Res. Commun. 253(1):33-37 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MEKK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1028-1058 amino acids from the C-terminal region of human MEKK6.
NCBI Gene ID #:
9064
NCBI Official Name:
Mitogen-activated protein kinase kinase kinase 6
NCBI Official Symbol:
MAP3K6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
143 kDa
Protein Accession #:
O95382
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
O95382
User NOte:
Optimal dilutions for each application to be determined by the researcher.