IPO9 Antibody
| Code | Size | Price |
|---|
| PSI-61-976-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Importin-9, Imp9, Ran-binding protein 9, RanBP9, IPO9, IMP9, KIAA1192, RANBP9
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
Background References:
- King, F.W., et al. Mol. Cell. Biol. 24(16):7091-7101(2004)
- Lubert, E.J., et al. Biochem. Biophys. Res. Commun. 303(3):908-913(2003)
- Jakel, S., et al. EMBO J. 21(3):377-386(2002)
- Muhlhausser, P., et al. EMBO Rep. 2(8):690-696(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IPO9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-106 amino acids from the N-terminal region of human IPO9.
NCBI Gene ID #:
55705
NCBI Official Name:
Importin-9
NCBI Official Symbol:
IPO9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
116 kDa
Protein Accession #:
Q96P70
Protein GI Number:
41688593
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96P70
User NOte:
Optimal dilutions for each application to be determined by the researcher.