TAF2 Antibody
| Code | Size | Price |
|---|
| PSI-64-211-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Transcription initiation factor TFIID subunit 2, 150 kDa cofactor of initiator function, RNA polymerase II TBP-associated factor subunit B, TBP-associated factor 150 kDa, Transcription initiation factor TFIID 150 kDa subunit, TAF(II)150, TAFII-150, TAFII150, TAF2, CIF150, TAF2B
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators.
Background References:
- Olsen, J.V., et al. Cell 127(3):635-648(2006)
- Kim, J.E., et al. J. Proteome Res. 4(4):1339-1346(2005)
- Guermah, M., et al. Mol. Cell 12(4):991-1001(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1180 amino acids from the C-terminal region of human TAF2.
NCBI Gene ID #:
6873
NCBI Official Name:
Transcription initiation factor TFIID subunit 2
NCBI Official Symbol:
TAF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
137 kDa
Protein Accession #:
Q6P1X5
Protein GI Number:
145559533
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q6P1X5
User NOte:
Optimal dilutions for each application to be determined by the researcher.