ATF5 Antibody

ProSci
Product Code: PSI-58-150
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-150-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human heart lysate Lane 4: mouse liver lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).

Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human heart lysate Lane 4: mouse liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

Further Information

Additional Names:
Cyclic AMP-dependent transcription factor ATF-5, cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx, ATF5, ATFX
Application Note:
For WB starting dilution is: 1:1000
Background:
Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.
Background References:
  • Wei, Y., et al. J. Biochem. 148(2):171-178(2010)
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Tomppo, L., et al. Biol. Psychiatry 65(12):1055-1062(2009)
  • Li, G., et al. Mol. Cancer Res. 7(6):933-943(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ATF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-216 amino acids from the C-terminal region of human ATF5.
NCBI Gene ID #:
22809
NCBI Official Name:
Cyclic AMP-dependent transcription factor ATF-5
NCBI Official Symbol:
ATF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
Q9Y2D1
Protein GI Number:
308153647
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9Y2D1
User NOte:
Optimal dilutions for each application to be determined by the researcher.