RPS12 Antibody

ProSci
Product Code: PSI-56-764
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-764-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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Western blot analysis in mouse kidney tissue lysates (35ug/lane).

Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western blot analysis in mouse kidney tissue lysates (35ug/lane).

Further Information

Additional Names:
40S ribosomal protein S12, RPS12
Application Note:
For WB starting dilution is: 1:1000
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Background References:
  • Yu, Y., et al. Protein Sci. 14(6):1438-1446(2005)
  • Andersen, J.S., et al. Nature 433(7021):77-83(2005)
  • Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
  • Sampath, P., et al. Mol. Cell. Biol. 23(5):1509-1519(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Pig, Rat, Xenopus
Immunogen:
This RPS12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human RPS12.
NCBI Gene ID #:
6206
NCBI Official Name:
40S ribosomal protein S12
NCBI Official Symbol:
RPS12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
15 kDa
Protein Accession #:
P25398
Protein GI Number:
224471878
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P25398
User NOte:
Optimal dilutions for each application to be determined by the researcher.