MED25 Antibody

ProSci
Product Code: PSI-55-085
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-085-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
2 / 3
Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates thdetected the MED25 protein (arrow).
3 / 3

Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates thdetected the MED25 protein (arrow).

Further Information

Additional Names:
Mediator of RNA polymerase II transcription subunit 25, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78, MED25, ACID1, ARC92, PTOV2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].
Background References:
  • Roupelieva, M., et al. J. Gen. Virol. 91 (PT 5), 1138-1149 (2010)Leal, A., et al. Neurogenetics 10(4):275-287(2009) Lee, H.K., et al. EMBO J. 26(15):3545-3557(2007) Sato, S., et al. Mol. Cell 14(5):685-691(2004) Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the N-terminal region of human MED25.
NCBI Gene ID #:
81857
NCBI Official Name:
Mediator of RNA polymerase II transcription subunit 25
NCBI Official Symbol:
MED25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
78 kDa
Protein Accession #:
Q71SY5
Protein GI Number:
158706143
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q71SY5
User NOte:
Optimal dilutions for each application to be determined by the researcher.