MYSM1 Antibody

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ProSci
Product Code: PSI-55-235
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-235-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
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Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Histone H2A deubiquitinase MYSM1, 2A-DUB, 3419-, Myb-like, SWIRM and MPN domain-containing protein 1, MYSM1, KIAA1915
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.
Background References:
  • Dephoure, N., et al. Proc. Natl. Acad. Sci. U.S.A. 105(31):10762-10767(2008)
  • Zhu, P., et al. Mol. Cell 27(4):609-621(2007)
  • Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
  • Yoneyama, M., et al. J. Mol. Biol. 369(1):222-238(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MYSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-139 amino acids from the N-terminal region of human MYSM1.
NCBI Gene ID #:
114803
NCBI Official Name:
Histone H2A deubiquitinase MYSM1
NCBI Official Symbol:
MYSM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
95 kDa
Protein Accession #:
Q5VVJ2
Protein GI Number:
74756898
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q5VVJ2
User NOte:
Optimal dilutions for each application to be determined by the researcher.