hGCN5 Antibody

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ProSci
Product Code: PSI-56-274
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-274-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
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Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Histone acetyltransferase KAT2A, General control of amino acid synthesis protein 5-like 2, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97, KAT2A, GCN5, GCN5L2, HGCN5
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).
Background References:
  • Terreni, M., et al. Retrovirology 7, 18 (2010) :
  • Atanassov, B.S., et al. Mol. Cell 35(3):352-364(2009)
  • Kelly, T.J., et al. J. Biol. Chem. 284(30):19945-19952(2009)
  • Mao, X., et al. Genes Dev. 23(7):849-861(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This hGCN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 807-837 amino acids from the C-terminal region of human hGCN5.
NCBI Gene ID #:
2648
NCBI Official Name:
Histone acetyltransferase KAT2A
NCBI Official Symbol:
KAT2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
94 kDa
Protein Accession #:
Q92830
Protein GI Number:
209572743
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q92830
User NOte:
Optimal dilutions for each application to be determined by the researcher.