HNF1A Antibody

ProSci
Product Code: PSI-63-609
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-609-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HNF1A Antibody in mouse liver tissue lysates (35ug/lane)
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Confocal immunofluorescent analysis of HNF1A Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of HNF1A Antibody in mouse liver tissue lysates (35ug/lane)
Confocal immunofluorescent analysis of HNF1A Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Further Information

Additional Names:
Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, LFB1, Transcription factor 1, TCF-1, HNF1A, TCF1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
HNF1A is a transcription factor required for the expression of several liver-specific genes. This protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Background References:
  • Bach,I., et.al., Genomics(1), 155-164 (1990)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This HNF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-205 amino acids from the Central region of human HNF1A.
NCBI Gene ID #:
6927
NCBI Official Name:
Hepatocyte nuclear factor 1-alpha
NCBI Official Symbol:
HNF1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
67 kDa
Protein Accession #:
P20823
Protein GI Number:
51338763
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P20823
User NOte:
Optimal dilutions for each application to be determined by the researcher.