DCLRE1B Antibody

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ProSci
Product Code: PSI-61-924
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-924-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the detected DCLRE1B protein (arrow).
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DCLRE1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the detected DCLRE1B protein (arrow).
DCLRE1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
5' exonuclease Apollo, 31--, DNA cross-link repair 1B protein, SNM1 homolog B, SNMIB, hSNM1B, DCLRE1B, SNM1B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]).
Background References:
  • Anders, M., et al. Cell Cycle 8(11):1725-1732(2009) Liu, L., et al. Cell Cycle 8(4):628-638(2009) Freibaum, B.D., et al. J. Biol. Chem. 283(35):23671-23676(2008) Bae, J.B., et al. Oncogene 27(37):5045-5056(2008) Demuth, I., et al. DNA Repair (Amst.) 7(8):1192-1201(2008) Matsuoka, S., et al. Science 316(5828):1160-1166(2007) Lenain, C., et al. Curr. Biol. 16(13):1303-1310(2006) Freibaum, B.D., et al. J. Biol. Chem. 281(22):15033-15036(2006) Ishiai, M., et al. Mol. Cell. Biol. 24(24):10733-10741(2004) Demuth, I., et al. Oncogene 23(53):8611-8618(2004) Dronkert, M.L., et al. Mol. Cell. Biol. 20(13):4553-4561(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DCLRE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-236 amino acids from the Central region of human DCLRE1B.
NCBI Gene ID #:
64858
NCBI Official Name:
5' exonuclease Apollo
NCBI Official Symbol:
DCLRE1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
Q9H816
Protein GI Number:
73620756
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9H816
User NOte:
Optimal dilutions for each application to be determined by the researcher.