SF3B1 Antibody

Contact us
ProSci
Product Code: PSI-57-247
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-247-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western Blot at 1:2000 dilution Lane 1: PANC-1 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: H-4-II-E whole cell lysate Lane 6: A431 whole cell lysate Lane 7: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western blot analysis in CEM cell line lysates (35ug/lane).
3 / 3
❮❯

Western Blot at 1:2000 dilution Lane 1: PANC-1 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: H-4-II-E whole cell lysate Lane 6: A431 whole cell lysate Lane 7: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, SF3b155, Spliceosome-associated protein 155, SAP 155, SF3B1, SAP155
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
Background References:
  • Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)
  • Tanuma, N., et al. J. Biol. Chem. 283(51):35805-35814(2008)
  • Pessa, H.K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8655-8660(2008)
  • Kuwasako, K., et al. Proteins 71(4):1617-1636(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus
Immunogen:
This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 amino acids from the N-terminal region of human SF3B1.
NCBI Gene ID #:
23451
NCBI Official Name:
Splicing factor 3B subunit 1
NCBI Official Symbol:
SF3B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
146 kDa
Protein Accession #:
O75533
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O75533
User NOte:
Optimal dilutions for each application to be determined by the researcher.