RPL23 Antibody

ProSci
Product Code: PSI-56-516
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-516-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
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RPL23 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
RPL23 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
60S ribosomal protein L23, 60S ribosomal protein L17, RPL23
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL17 because the encoded protein shares amino acid identity with ribosomal protein L17 from Saccharomyces cerevisiae; however, its official symbol is RPL23. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Background References:
  • Gehring, N.H., et al. Cell 137(3):536-548(2009) Wanzel, M., et al. Nat. Cell Biol. 10(9):1051-1061(2008) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005) Jin, A., et al. Mol. Cell. Biol. 24(17):7669-7680(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Zebrafish, Pig, Rat
Immunogen:
This RPL23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the Central region of human RPL23.
NCBI Gene ID #:
9349
NCBI Official Name:
60S ribosomal protein L23
NCBI Official Symbol:
RPL23
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
15 kDa
Protein Accession #:
P62829
Protein GI Number:
51338639
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P62829
User NOte:
Optimal dilutions for each application to be determined by the researcher.