BRD3 Antibody

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ProSci
Product Code: PSI-63-366
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-366-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Saos-2 cell lysate (Lane 2).
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Antibody is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Saos-2 cell lysate (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Bromodomain-containing protein 3, RING3-like protein, BRD3, KIAA0043, RING3L
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.
Background References:
  • Kaneko, H., et al.,Investig Allergol Clin Immunol 12(2):86-90 (2002).
  • Thorpe, K.L., et al., Immunogenetics 44(5):391-396 (1996).
  • Thorpe, K.L., et al., Gene 200 (1-2), 177-183 (1997).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This BRD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-702 amino acids from the C-terminal region of human BRD3.
NCBI Gene ID #:
8019
NCBI Official Name:
Bromodomain-containing protein 3
NCBI Official Symbol:
BRD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
Q15059
Protein GI Number:
12643726
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q15059
User NOte:
Optimal dilutions for each application to be determined by the researcher.