GLIS1 Antibody

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ProSci
Product Code: PSI-63-685
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-685-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of GLIS1 Antibody in mouse stomach tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with GLIS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of GLIS1 Antibody in mouse stomach tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with GLIS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Zinc finger protein GLIS1, GLI-similar 1, GLIS1 (HGNC:29525)
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription (Kim et al., 2002 [PubMed 12042312]).
Background References:
  • Kim,Y.S., et.al., Nucleic Acids Res. 31 (19), 5513-5525 (2003)
  • Nakanishi,G., et.al., J. Invest. Dermatol. 126 (1), 49-60 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GLIS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-161 amino acids from the N-terminal region of human GLIS1.
NCBI Gene ID #:
148979
NCBI Official Name:
Zinc finger protein GLIS1
NCBI Official Symbol:
GLIS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q8NBF1
Protein GI Number:
209572624
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8NBF1
User NOte:
Optimal dilutions for each application to be determined by the researcher.