RPL27 Antibody

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ProSci
Product Code: PSI-56-865
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-865-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:4000 dilution + RPMI 8226 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in mouse lung tissue lysates (35ug/lane).
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Western Blot at 1:4000 dilution + RPMI 8226 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse lung tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
60S ribosomal protein L27, RPL27
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Background References:
  • Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
  • Andersen, J.S., et al. Nature 433(7021):77-83(2005)
  • Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
  • Mazumder, B., et al. Cell 115(2):187-198(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Zebrafish, Monkey, Pig, Rat
Immunogen:
This RPL27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the C-terminal region of human RPL27.
NCBI Gene ID #:
6155
NCBI Official Name:
60S ribosomal protein L27
NCBI Official Symbol:
RPL27
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
P61353
Protein GI Number:
47117772
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P61353
User NOte:
Optimal dilutions for each application to be determined by the researcher.