POLR1C Antibody

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ProSci
Product Code: PSI-61-539
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-539-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: HepG2 whole cell lysate Lane 7: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with POLR1C antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: HepG2 whole cell lysate Lane 7: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with POLR1C antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit C, RNA polymerases I and III subunit AC1, AC40, DNA-directed RNA polymerases I and III 40 kDa polypeptide, RPA40, RPA39, RPC40, POLR1C, POLR1E
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:10~50
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Background References:
  • Rush,J., Nat. Biotechnol. 23 (1), 94-101 (2005)
  • Hirschler-Laszkiewicz,I., J. Biol. Chem. 278 (21), 18953-18959 (2003)
  • Dammann,R., Biochim. Biophys. Acta 1396 (2), 153-157 (1998)
  • Seither,P., Chromosoma 106 (4), 216-225 (1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POLR1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-346 amino acids from the C-terminal region of human POLR1C.
NCBI Gene ID #:
9533
NCBI Official Name:
DNA-directed RNA polymerases I and III subunit RPAC1
NCBI Official Symbol:
POLR1C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
O15160
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
O15160
User NOte:
Optimal dilutions for each application to be determined by the researcher.