ALKBH3 Antibody

Contact us
ProSci
Product Code: PSI-63-586
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-586-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis of ALKBH3 Antibody in mouse kidney tissue lysates (35ug/lane)
2 / 2
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ALKBH3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Western blot analysis of ALKBH3 Antibody in mouse kidney tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ALKBH3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, 11411-, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, ALKBH3, ABH3, DEPC1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.
Background References:
  • Sundheim,O., et.al., EMBO J. 25 (14), 3389-3397 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ALKBH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the C-terminal region of human ALKBH3.
NCBI Gene ID #:
221120
NCBI Official Name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
NCBI Official Symbol:
ALKBH3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
Q96Q83
Protein GI Number:
74752087
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96Q83
User NOte:
Optimal dilutions for each application to be determined by the researcher.