GCNT2 Antibody

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ProSci
Product Code: PSI-55-091
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-091-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
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GCNT2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
GCNT2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase, isoform B, N-acetylglucosaminyltransferase, I-branching enzyme, IGNT, GCNT2, GCNT5, II, NACGT1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described.
Background References:
  • Twu, Y.C., et al. Blood 110(13):4526-4534(2007)
  • Wang, L., et al. Biochem. Biophys. Res. Commun. 331(4):958-963(2005)
  • Pras, E., et al. Invest. Ophthalmol. Vis. Sci. 45(6):1940-1945(2004)
  • Inaba, N., et al. Blood 101(7):2870-2876(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GCNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-391 amino acids from the C-terminal region of human GCNT2.
NCBI Gene ID #:
2651
NCBI Official Name:
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase, isoform B
NCBI Official Symbol:
GCNT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q06430
Protein GI Number:
298351849
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q06430
User NOte:
Optimal dilutions for each application to be determined by the researcher.