EEFSEC Antibody

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ProSci
Product Code: PSI-63-770
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-770-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of EEFSEC Antibody in mouse lung tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EEFSEC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of EEFSEC Antibody in mouse lung tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EEFSEC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Selenocysteine-specific elongation factor, Elongation factor sec, Eukaryotic elongation factor, selenocysteine-tRNA-specific, EEFSEC, SELB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
EEFSEC is a translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EEFSEC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-568 amino acids from the C-terminal region of human EEFSEC.
NCBI Gene ID #:
60678
NCBI Official Name:
Selenocysteine-specific elongation factor
NCBI Official Symbol:
EEFSEC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
P57772
Protein GI Number:
259016384
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P57772
User NOte:
Optimal dilutions for each application to be determined by the researcher.