HIST1H2AG Antibody

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ProSci
Product Code: PSI-61-041
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-041-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: L929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
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Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody . was diluted at 1:1000 at each lane.
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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: L929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody . was diluted at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:25

For FACS starting dilution is: 1:25
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Background References:
  • Albig W.,et al.Hum. Genet. 101:284-294(1997).
  • Albig W.,et al.Biol. Chem. 380:7-18(1999).
  • Dobner T.,et al.DNA Seq. 1:409-413(1991).
  • Mannironi C.,et al.DNA Cell Biol. 13:161-170(1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Bovine, Xenopus, Yeast, Chicken, Monkey, Zebrafish, Drosophila
Immunogen:
This HIST1H2AG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-87 amino acids from the Central region of human HIST1H2AG.
NCBI Gene ID #:
8329, 8330, 8332, 8336, 8969
NCBI Official Name:
Histone H2A type 1
NCBI Official Symbol:
HIST1H2AG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
P0C0S8
Protein GI Number:
83288406
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P0C0S8
User NOte:
Optimal dilutions for each application to be determined by the researcher.