PARS2 Antibody

ProSci
Product Code: PSI-55-619
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-619-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Western blot analysis of PARS2 using rabbit polyclonal PARS2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PARS2 gene.
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Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Western blot analysis of PARS2 using rabbit polyclonal PARS2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PARS2 gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Probable proline--tRNA ligase, mitochondrial, Prolyl-tRNA synthetase, ProRS, PARS2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
Background References:
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Bonnefond, L., et al. Biochemistry 44(12):4805-4816(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-417 amino acids from the C-terminal region of human PARS2.
NCBI Gene ID #:
25973
NCBI Official Name:
Probable proline--tRNA ligase, mitochondrial
NCBI Official Symbol:
PARS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
Q7L3T8
Protein GI Number:
73919761
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q7L3T8
User NOte:
Optimal dilutions for each application to be determined by the researcher.