APOBEC2 Antibody

ProSci
Product Code: PSI-62-013
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-013-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the detected APOBEC2 protein (arrow).
2 / 3
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
3 / 3

Western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the detected APOBEC2 protein (arrow).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Probable C->U-editing enzyme APOBEC-2, 354-, APOBEC2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100
Background:
APOBEC2 belongs to the cytidine and deoxycytidylate deaminase family. It is probable C to U editing enzyme whose physiological substrate is not yet known. It does not display detectable apoB mRNA editing and has a low intrinsic cytidine deaminase activity.
Background References:
  • Liao, W., et al. Biochem. Biophys. Res. Commun. 260(2):398-404(1999)
  • Oka, K., et al. J. Biol. Chem. 272(3):1456-1460(1997)
  • Lau, P.P., et al. Proc. Natl. Acad. Sci. U.S.A. 91(18):8522-8526(1994)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This APOBEC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from the N-terminal region of human APOBEC2.
NCBI Gene ID #:
10930
NCBI Official Name:
Probable C->U-editing enzyme APOBEC-2
NCBI Official Symbol:
APOBEC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
Q9Y235
Protein GI Number:
23396446
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9Y235
User NOte:
Optimal dilutions for each application to be determined by the researcher.