TDRD7 Antibody

ProSci
Product Code: PSI-55-541
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-541-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
3 / 4
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Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Tudor domain-containing protein 7, PCTAIRE2-binding protein, Tudor repeat associator with PCTAIRE-2, Trap, TDRD7, PCTAIRE2BP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
TDRD7, also known as Tudor domain containing protein 7, is a component of chromatoid bodies in spermatids (named processing bodies or P-bodies in somatic cells). It is found in a mRNP complex together with TDRD1, TDRD6 and DDX4. It is also found in a complex with CABLES1, PCTK2 and PIWIL1. TDRD7 has been shown to interact with histone H3 tri-methylated at K9 in vitro.
Background References:
  • Lim, J., et al. Cell 125(4):801-814(2006)
  • Conte, N., et al. Oncogene 22(50):8102-8116(2003)
  • Lauffart, B., et al. Biochem. J. 363 (PT 1), 195-200 (2002) :
  • Yamochi, T., et al. Biochem. Biophys. Res. Commun. 286(5):1045-1050(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This TDRD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 807-835 amino acids from the C-terminal region of human TDRD7.
NCBI Gene ID #:
23424
NCBI Official Name:
Tudor domain-containing protein 7
NCBI Official Symbol:
TDRD7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
124 kDa
Protein Accession #:
Q8NHU6
Protein GI Number:
152031705
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8NHU6
User NOte:
Optimal dilutions for each application to be determined by the researcher.