CHPF Antibody

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ProSci
Product Code: PSI-63-759
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-759-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CHPF Antibody in MDA-MB435, MCF-7, HepG2, A375 cell line and mouse testis tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human testis tissue reacted with CHPF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of CHPF Antibody in MDA-MB435, MCF-7, HepG2, A375 cell line and mouse testis tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with CHPF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Chondroitin sulfate synthase 2, Chondroitin glucuronyltransferase 2, Chondroitin-polymerizing factor, ChPF, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II, N-acetylgalactosaminyltransferase 2, CHPF, CSS2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
CHPF is a protein that has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.
Background References:
  • Matsuoka,S., et.al., Science 316 (5828), 1160-1166 (2007)
  • Colland,F., et.al., Genome Res. 14 (7), 1324-1332 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CHPF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-354 amino acids from the Central region of human CHPF.
NCBI Gene ID #:
79586
NCBI Official Name:
Chondroitin sulfate synthase 2
NCBI Official Symbol:
CHPF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
Q8IZ52
Protein GI Number:
311033361
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8IZ52
User NOte:
Optimal dilutions for each application to be determined by the researcher.